Regarding the TCGA analysis showing downregulation of PIK3R1 (a regulatory subunit that often acts as a tumor suppressor by inhibiting PI3K activity), we hypothesize that puerarin may upregulate PIK3R1 expression or function, or alternatively target the catalytic p110 subunits, to modulate the pathway despite this downregulation in tumors. The gene discussed is PIK3R1; the disease is neoplasm.