GSK3B and Alzheimer disease: Two-way ANOVA indicated a genotype effect on protein expression of GSK-3β (ser 9), PI3K, and GSK3β; thus, Western blot analysis revealed that transgenic 3×Tg-AD mice only exhibited a marked phosphorylation of GSK-3β at serine 9, as a basal phenotypic finding (Figure 5A,C,E,G).