To generate a murine model of hyper-thyroidism, the extracellular A-subunit of the human TSH receptor (TSHR; amino acid residues 1–289) was amplified by PCR from human TSHR cDNA and sub-cloned into an adenoviral vector (Ad/TSHR) constructed by VectorBuilder (Chicago, IL, USA). This evidence concerns the gene TSHR and hyperthyroidism.