When discriminating between mild and moderate depression, NLR (cutoff > 2.22) had an AUC of 0.744 (95% CI: 0.593–0.863), while serum ANXA3 (cutoff ≤ 11.3) had an AUC of 0.917 (95% CI: 0.795–0.978) with perfect (100%) specificity. This evidence concerns the gene ANXA3 and major depressive disorder.