When dividing patients by the type of mutation in the GBA1 gene, we found that in samples from GBA1-PD (p.N370S) patients, the EV diameters of double vesicles, 116.8 (53.3–450.5) nm, and multilayer, 219.0 (83.0–491.3) nm, vesicles were larger compared to those in the control group, 88.4 (44.4–138.0) nm and 196.3 (103.7–274.6) nm (p = 0.0029 and p = 0.024, respectively) (Figure 3D). Here, GBA1 is linked to Parkinson disease.