EVs for proteomic profiling were isolated from 15 mL of plasma (10 individuals in each group (GBA1-PD without division by type of mutation in the GBA1 gene and controls)) using ultracentrifugation separately for each individual according to the EV purification protocol, and then EV samples were pooled from each five individuals per one pool. The gene discussed is GBA1; the disease is Parkinson disease.