To investigate the role of MYH9 in DEV pathogenesis in vivo, we conducted a duck infection study following the experimental schema outlined in Figure 8A. Two-month-old female ducks were intranasally (i.n.)administered PBS or BLEB at 0.004 mg/kg or 0.010 mg/kg either pre-infection (24 h prior) or post-infection (6 h after) with the virulent CHv strain of DEV. The gene discussed is MYH9; the disease is infection.