Considering the pivotal role of G6PD in tumor metabolism and survival, we investigated the pharmacological effects of nine synthetic compounds, including seven benzimidazole-based derivatives, one nitrothiazole derivative, and a phenylpropanoic acid analog on glioblastoma cell lines (A172 and U87-MG) under both normoxic and hypoxic conditions. This evidence concerns the gene G6PD and neoplasm.