To determine the extent to which intracellularly suppressed CYR61 regulates PCa cell migration, we transiently reduced CYR61 protein expression levels (see verification of CYR61 suppression; Figure 1A) in PC3 and LNCaP cell lines and analyzed cell migration using a 2D Transwell co-culture migration assay. Here, CCN1 is linked to posterior cortical atrophy.