In MASLD development pathways, the dysregulation of AMP-activated protein kinase (AMPK), mechanistic target of rapamycin (mTOR), and Sirtuin 1 (SIRT1) plays an important role due to their functions in glucose and lipid metabolism, lipid and protein synthesis, inflammation, and autophagy. This evidence concerns the gene MTOR and metabolic dysfunction-associated steatotic liver disease.