The level of active MMP-9 in serum was measured using the Fluorokine E assay, and the mean (50.6 ng/mL ± 12.4 ng/mL) level in ALS serum was found to be significantly higher (p < 0.0001, Cohen’s d = 2.47) as compared to HC (20.97 ng/mL ± 11.29 ng/mL), with a mean increase of 2.4-fold (Figure 10). The gene discussed is MMP9; the disease is amyotrophic lateral sclerosis.