This problem originally also occurred after transient depletion of PEX3, Sec62, and Sec63 and was the reason for the use of CRISPR/Cas9-mediated Sec62- as well as Sec63-knock-out cells or fibroblasts from PEX3-deficient Zellweger Syndrome patients [98,157]. The gene discussed is SEC63; the disease is Zellweger syndrome.