Moreover, an in vivo study with supplementation with 40 mg/kg per day in C57BL/6 mice of lycopene shows an increase in the CD4+/CD8+ T-cell ratio and in the percentages of IFNγ+, perforin+, and granzyme B+ expression on CD8+ T cells and an enhanced apoptosis in tumor cells with combined lycopene and anti-PD-1 treatment in comparison with those of either lycopene or anti-PD-1 alone [59]. The gene discussed is CD8A; the disease is neoplasm.