To investigate the biological function of PARP3 in AML cells, the lentiviruses for PARP3 shRNA and the empty vector transfected into the MOLM13 and THP-1 cells and the knockdown efficiency was examined using RT-qPCR and WB (Figure 2A; the original western blots images are shown in Supplementary Figure S2). The gene discussed is PARP3; the disease is acute myeloid leukemia.