To further explore this concern, Quarta et al. investigated optimal tumor-to-background (TtB) ratio thresholds (≥2 vs. ≥3 vs. ≥4) in a prospective study comparing intraoperative findings with PSMA PET imaging, recommending a TtB threshold assessment of ≥2 to optimize sensitivity and minimize the risk of missing true positive lesions [66]. The gene discussed is FOLH1; the disease is neoplasm.