These genes were further subjected to LASSO-penalized Cox regression (Figure 1d,e) and multivariate Cox regression, yielding 5 final genes—IFI27, KIF20A, KLK10, SPINK7, and TOP2A (Figure S3)—all of which exhibited higher expression levels in PAAD tissues compared to normal pancreatic tissues. This evidence concerns the gene IFI27 and pancreatic adenocarcinoma.