The clinicopathological relevance analysis of the 5-gene signature with tumor staging revealed distinct associations: IFI27 demonstrated positive associations with Stage I–II tumors, lymph node metastasis, and T1/T3 stages; KLK10 exhibited positive correlations with Stage I–II tumors and T2/T3 stages; KIF20A showed significant linkages to Stage I–II tumors and T2/T3 stages; SPINK7 exclusively correlated with T1, T2, and T4 stages; and TOP2A was uniquely associated with T2 and T3 stages (Figure S5). This evidence concerns the gene TOP2A and neoplasm.