Large validation cohorts confirmed this: pyrosequencing of samples from Chinese (PCa n = 339) and German (PCa n = 328) cohorts identified CRMP4 methylation as an independent predictor of lymph node metastasis (cohort 1 HR = 8.4; cohort 2 HR = 12.5; both p < 0.001) [64]. This evidence concerns the gene DPYSL3 and posterior cortical atrophy.