Similarly, CAMK2N1, a well-established tumor suppressor gene (TSG) implicated in PCa, was found to be downregulated via promoter hypermethylation in a subset of PCa cell lines and in patient samples (both TCGA database and local cohort: pyrosequencing; BPH n = 16, PCa n = 52) compared to the normal prostate. This evidence concerns the gene CAMK2N1 and benign prostatic hyperplasia.