Lactate overproduction during hyperglycemia promoted the AARS1-mediated lactylation of YTHDC1 at lysine 82 (K82), which enhanced the binding of E3 ubiquitin ligase ring finger protein 183 (RNF183) to YTHDC1, leading to the ubiquitination and proteasomal degradation of YTHDC1 in bladder cancer (BC) cell lines (HT1376 and RT112 cells). Here, RNF183 is linked to urinary bladder carcinoma.