Importantly, immunohistochemistry on myocardial infarction mouse models demonstrated that the anti-VGLL3 avian antibody selectively stains fibrotic tissues, with significantly stronger signals in myocardial infarction hearts compared to controls (p < 0.001; Figure 4F,G), confirms its in situ binding specificity and potential for tissue-level fibrosis detection. This evidence concerns the gene VGLL3 and myocardial infarction.