Studies on HFD-induced obese mice and HepG2 cells have demonstrated that Pueraria flavonoids activate autophagy via the PI3K/Akt/mTOR signaling pathway, thereby reducing intracellular lipid accumulation and inflammation and alleviating the pathological progression of non-alcoholic fatty liver disease (NAFLD) [17]. Here, MTOR is linked to metabolic dysfunction-associated steatotic liver disease.