In addition to causing dyslipidemia, HFD-induced obesity can lead to varying degrees of liver injury, including elevated serum ALT and AST levels, hepatic fat deposition, hepatocyte vacuolization, sinusoidal dilation, and inflammatory cell infiltration—pathological features of non-alcoholic fatty liver disease (NAFLD) [38]. Here, GPT is linked to obesity due to melanocortin 4 receptor deficiency.