This computational workflow could distinguish tumor tissue from non-tumor, identify Raman peaks related to DNA and proteins more intense in tumors, differentiate between IDH1-mutated and IDH1-wild-type gliomas, and identify specific methylation subtypes, notably distinguishing cytosine-phosphate-guanine island methylator phenotype(G-CIMP)-high from G-CIMP-low within IDH1-mutated tumors. Here, IDH1 is linked to glioma.