The differential analysis of HTR1F expression in pan-cancer revealed that HTR1F expression was significantly different in the gender subgroups of five tumor types (LGG, LAML, KIRP, KIPAN, and READ), histological grade in six tumor types (GBMLGG, LGG, ESCA, STES, STAD, and OV), and pathological stage in four tumor types (KIRP, KIPAN, THYM, and LIHC) (Supplemental Figure S1A–C). The gene discussed is HTR1F; the disease is neoplasm.