We evaluated B7-H3 expression across three neuroblastoma cell lines (LAN-1, IMR-32, and SK-N-BE(2)), an osteosarcoma line (143B), a T cell line (Jurkat), B cell lines (Daudi and IM-9), and a myeloid cell line (THP1) via flow cytometry (Figure 2B). Here, CD276 is linked to osteosarcoma.