Since EAU development involves Th1 and Th17 cell differentiation, which are crucial for the pathogenesis of EAU, while Tregs suppress EAU development, we analyzed splenic CD4+ T cells, including Th1 and Th17 cells, in WT-EAU and STZ-EAU mice (WT and STZ-induced diabetic mice immunized with hIRBP-p) using flow cytometry. The gene discussed is CD4; the disease is diabetes mellitus.