Some signaling pathways that changed in terms of gene expression in the presence of 2S-13 in the 4T1 cell line are pathways in cancer, the MAPK signaling pathway, apoptosis, the PI3K-Akt signaling pathway, and then the PI3K-Akt signaling pathway, as well as the RAP1 and cell cycle pathways (Figure 8 and Figure 9). This evidence concerns the gene AKT1 and cancer.