METTL16 G110C, which was identified in intestinal cancer, did not significantly alter binding to U6 snRNA; however, the KD2 value for SAM binding was greater than 1000 μM and the observed rate constant at 1 mM SAM was barely measurable: 0.005 ± 0.003 min−1 (Table 5 and Figure S4B). This evidence concerns the gene METTL16 and intestinal cancer.