Behavioral assessments (Novel Object Recognition, Morris Water Maze) quantified cognitive impairment in scopolamine-treated mice, complemented by histological (Nissl staining, BDNF IHC), biochemical (ACh, AChE, BChE), and molecular (BDNF, CREB) analyses of hippocampal and cortical tissues. This evidence concerns the gene ACHE and Cognitive impairment.