Behavioral assessments (Novel Object Recognition, Morris Water Maze) quantified cognitive impairment in scopolamine-treated mice, complemented by histological (Nissl staining, BDNF IHC), biochemical (ACh, AChE, BChE), and molecular (BDNF, CREB) analyses of hippocampal and cortical tissues. The gene discussed is BCHE; the disease is Cognitive impairment.