Further, PD-L1 levels were found to be elevated in post-chemotherapy samples [100], suggesting that chemotherapy may play a role in rendering RMS tumors more immunogenic or “hot.” A study by Kim et al. evaluated the prognostic significance of PD-L1 expression in several types of soft tissue sarcomas, including 32 RMS patients [101]. The gene discussed is CD274; the disease is soft tissue sarcoma.