Within asthmatic patients, 11 of these differentially methylated regions (DMRs) correlated with serum immunoglobulin E (IgE) levels and 16 with percent-predicted FEV1, and integrative LCMix analysis uncovered 2484 high-confidence methylation–expression pairs (including IL4, RUNX3, ST2), with pyrosequencing validation confirming asthma-associated methylation at RUNX3, IL4, CAT, kruppel-like factor 6 (KLF6), and NBR2 loci [66]. This evidence concerns the gene IGHE and asthma.