Combining NK cells with Avastin® and irinotecan enhanced cytotoxicity in vitro and prolonged survival in intracranial PDXs harboring ecDNA-driven MYC and PDGFRA amplifications.<h4>Conclusion</h4>Intracranial PDX models were successfully established from cryopreserved GBM tissues through subcutaneous expansion. The gene discussed is MYC; the disease is glioblastoma.