Moreover, USP13 deficiency selectively prevented IL-1β production, as well as pro–caspase-1 and pro–IL-1β cleavage, triggered by the NLRP3 inflammasome–specific activators but not the AIM2 (absent in melanoma 2) inflammasome activator flagellin and the NLRC4 inflammasome activator poly(deoxyadenylic-deoxythymidylic) acid sodium salt [poly(dA:dT)] in LPS-primed BMDMs (Fig. 5, G and H), which indicates that USP13 specifically regulates NLRP3 inflammasome activation. This evidence concerns the gene NLRC4 and melanoma.