Min Liu et al. identified a phosphotyrosine (pY)-containing peptide derived from nephrin/Tir similar sequence, promoted Nck/N-WASP LLPS, functionally mimicking the role of phosphorylated nephrin [97], while peptide p1, a covalent blocker targeting the Nck-SH2 domain at Lys331, was designed to effectively disrupts condensates formation and prevents bacterial infection in intestinal epithelial cells [97]. Here, NCK1 is linked to bacterial infectious disease.