To elucidate the potential mechanisms by which TRNT1 exerts its effects in BC cells, we conducted RNA‐seq analysis on SK‐BR‐3 cells overexpressing TRNT1 (OE‐TRNT1) versus vector control and on MCF‐7 cells with TRNT1 knockdown (sh‐TRNT1) versus non‐targeting control (sh‐NC). The gene discussed is TRNT1; the disease is breast cancer.