I-κBα phosphorylation was found diminished by Asiaticoside in human CRC cell lines (HCT116, SW480, LoVo) concentration-dependently, which effectively blocks the nuclear translocation of the P65 subunit, indicating its potential to suppress colorectal tumor progression and stimulate apoptotic mechanisms through NF-κB pathway inhibition. This evidence concerns the gene NFKB1 and colorectal carcinoma.