Validation experiments in this study proved that the levels of EMP1, FGFR1, PLPPR4 and JUN were obviously decreased in BLCA tissues and cell lines (T24 and J82), while TNFRSF25 showed an opposite trend, compared with those in para-cancerous normal tissues and normal urothelial SV-HUC-1 cells. This evidence concerns the gene JUN and bladder transitional cell carcinoma.