MYCN and neuroblastoma: Furthermore, RNA sequencing (RNA-seq) and Western blot analyses, combined with Transwell invasion assays, revealed that ARID1A depletion in MYCN-driven NB induces a phenotypic shift from the differentiated adrenergic (ADRN) state to a more aggressive mesenchymal (MES) state, as evidenced by altered expression of lineage-specific markers and enhanced tumor invasiveness (12, 23).