Moreover, we also presented (i) the first use of targeted long-read sequencing in the field of myotonic dystrophy, to our knowledge, to assess Mbnl1-dependent transcript isoform changes for a gene with complex splice patterning, (ii) a novel method for the assessment of mechanical and electrical myotonia in DM1 mouse models, and (iii) a comprehensive analysis of the histopathological and transcriptomic impact of myotonia in skeletal muscle of DM1 mouse models. This evidence concerns the gene MBNL1 and Myotonia.