With respect to the association between ACh and liver diseases, some reports have shown that, in the liver, ACh and the activation of α7‐nAChR signaling in resident hepatic cells, such as hepatocytes, Kupffer cells, and satellite cells, inhibit inflammation and alleviate abnormal lipid metabolism in NAFLD.[49, 50, 51] However, in this study, we reported that irregular exercise increased hepatic ACh levels, which was accompanied by the infiltration of a novel FBXL6high neutrophil subset. The gene discussed is CHRNA7; the disease is metabolic dysfunction-associated steatotic liver disease.