Moreover, treating the CAF8 Gy‐CM with RNase and Triton X‐100 led to a significantly reduced miR‐193a‐3p level in CM‐treated ESCC cells compared to treatment with RNase alone (Figure 2H), suggesting that EV mediates the intercellular transfer of miR‐193a‐3p from irradiated CAFs to ESCC cells. This evidence concerns the gene TBX1 and esophageal squamous cell carcinoma.