The ROC curve (Fig. 8B-D) manifested that HG PCK1 level had high accuracy for the classification of hemorrhoids and control samples (area under the curve (AUC) > 0.9), and the expression levels of ALDOB, PCK2, PFKL, PKM, ENO1, and FBP1 demonstrated a moderate level of accuracy (0.7 < AUC < 0.9) in distinguishing between hemorrhoids and anal fissures (controls) (AUC confidence interval in ROC analysis is provided as a Supplementary file). This evidence concerns the gene PFKL and hemorrhoid.