The image-based cytotoxic assay also showed that 212Pb-ADVC001 displayed potent cytotoxic activity against PSMA-positive prostate cancer cells with an average EC50 of 4.53 ± 0.71 kBq/mL (n = 3) and 10.42 ± 2.37 kBq/mL (n = 3) for PC3-PIP and C4-2 cells, respectively, whereas the EC50 was not calculated for PC3 because of the low level of cell death (Fig. 2B; Supplemental Fig. 2). The gene discussed is FOLH1; the disease is Familial prostate cancer.