We adapted this methodology to create a mouse model of focal DEPDC5-related epilepsy without neuronal migration defects via unilateral stereotactic injection of AAV-Cre-GFP into the motor cerebral cortex of postnatal day zero (P0) or P1 Depdc5-floxed (Depdc5c/c or Depdc5c/–) mouse pups. This evidence concerns the gene DEPDC5 and epilepsy.