GPT and metabolic dysfunction-associated steatotic liver disease: Liver function markers, such as ALT and AST, and blood lipid markers, including total cholesterol (TC) and triglyceride (TG) levels, were significantly elevated in the HFD and db/db groups compared to those in the CD and db/m groups (Figure 2G,J) (Figure S2G,H), confirming the successful establishment of the NAFLD model.