Previous studies have shown that ANGPTL8 is highly expressed in steatotic liver cells and binds to PIRB receptors on macrophages, promoting liver injury and fibrosis.[23, 42] However, the interaction between ANGPTL8 and PIRB, as well as its relationship with ALOX5AP and linoleic acid metabolism in mice with NAFLD and concurrent renal fibrosis, has not been thoroughly investigated. This evidence concerns the gene ANGPTL8 and metabolic dysfunction-associated steatotic liver disease.