IVM in primary breast cancer and lung metastasis sites revealed that tyrosine kinase with immunoglobulin-like and EGF-like domains 2 (Tie2) high macrophages and mammalian-enabled (MENA)-overexpressing tumor cells directly contact endothelial cells near blood vessels, forming specialized tumor microenvironment metastatic portals (TMEMs). The gene discussed is TEK; the disease is neoplasm.