Immunohistochemical, western blot, and multiplex analysis showed that the TLR activation induced the expression of psoriasis associated markers like S100A7, p-STAT3, CXCL-1, IL-8, IL-1α, S100A9, and IL-23 in the wild type but not in the TLR KO epidermis models. The gene discussed is STAT3; the disease is psoriasis.