By employing the principles of Mendelian inheritance, Mendelian randomization (MR) mitigates the effects of measurement errors, confounding variables, and reverse causality.[9] Here, we investigated the causal relationship between cholecystitis and CD45 in CD33br HLA-DR+ immune cells, as well as the mediating role of glycine levels in this association. The gene discussed is PTPRC; the disease is Cholecystitis.