In order to further elucidate the pivotal function of ubiquitination-related genes in ovarian cancer, significant interactions between the eight core proteins included in the ubiquitination-associated prognostic model were identified by protein interaction network PPI analysis (Figure 4A), namely TRAF4, UBE2L3, FBXO45, UBE2L6, FBXL14, SKP2, CHAF1B, WDR77 The protein with the highest impact (HR 1.0615) in univariate regression based on the above proteins was selected for further validation. The gene discussed is FBXO45; the disease is ovarian cancer.