Functional enrichment analysis of gene sets with genes that have increased chromatin accessibility and increased mRNA expression in MLS identified gene sets connected to PRC2, RB1 and SMAD2/3 targets, mesenchymal breast cancer, cell migration, and biological adhesion, while in EWS, enriched gene sets included EWSR1::FLI1, PRC2 complex and H3K27me3 targets, as well as neurogenesis and cell signaling processes (Fig. 3F, G). The gene discussed is SMAD2; the disease is breast carcinoma.