The aim of this study was to evaluate the potential of dynamic CEUS quantification analysis using Vuebox® software in preoperative distinguishing MTM subtype and high Ki-67 pattern in HCC patients, and to develop a prediction model based on clinical data, CEUS features and relative quantification parameters to identify MTM-HCC as well as high Ki-67 pattern. Here, MKI67 is linked to hepatocellular carcinoma.