PTK7 and non-small cell lung carcinoma: To further determine the correlation between efficacy and target expression, we evaluated the antitumor activity of Sgc8c-M in PTK7-negative A549 NSCLC models (Supplementary Fig. 10).22 Binding assays confirmed minimal binding of Cy5-Sgc8c-M to A549 cells compared to the Cy5-Ctrl-M control (Supplementary Fig. 10b).