Following challenge with np-Der p1 protein, immunized mice exhibited reduced allergic responses, including lessened eosinophilia, decreased Th2 (GATA3+ and IL-5+IL-13+) and Th17 (IL-17A+) cell frequencies, and lowered mucus production (Figure 5, C–E). This evidence concerns the gene GATA3 and Increased total eosinophil count.